高、低脂鸡脂肪组织ATGL基因的表达及其激素调控的差异分析

doi:10.11843/j.issn.0366-6964.2013.12.003

Abstract

Abstract:

This study aimed to investigate the function of adipose triglyceride lipase（ATGL）in chicken fat deposition, and to analyze the effects of adrenaline and glucagon on the gene expression of ATGL in adipose tissue and adipocytes of the fat and lean broilers. The tissue expression pattern of ATGL gene was analyzed by semi-quantitative RT-PCR (SqRT-PCR). The expression of ATGL gene and its activator Comparative gene identification 58 (CGI-58) gene in abdominal adipose tissue and adipocytes in the fat and lean broiler lines of the 16th generation of NEAUHLF at 1-7 week-old was analyzed by Real-time RT-PCR. The results showed that chicken ATGL gene was highly expressed in abdominal fat, subcutaneous fat, crop fat, kidney, liver and gizzard fat. On the whole, the mRNA expression levels of ATGL and CGI-58 genes were higher in the lean broiler lines than that in the fat broiler lines. In particular, the expression of ATGL and CGI-58 genes in the lean broiler lines was significantly higher than that in the fat broiler lines at 2 week-old (P＜0.05). Adrenaline and glucagon up-regulated the expression of ATGL gene in the abdominal adipose tissue and adipocytes of the fat broiler lines and promoted lipolysis, which indicated that the fat broiler lines were more sensitive to the both hormones than the lean broiler lines. This study not only laid the foundation for revealing the difference of the fat phenotype of fat and lean broiler lines, but also offered a basis for clarifying the function of ATGL in fat deposition in broiler.

CLC Number:

S831.2

SHI Ming-xin, RONG En-guang, ZHOU Wei-nan, SHI Hong-yan, LI Hui, WANG Ning. Adipose Tissue Expression and Hormonal Regulation of ATGL Gene in the Fat and Lean Broiler Lines[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1874-1881.

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Adipose Tissue Expression and Hormonal Regulation of ATGL Gene in the Fat and Lean Broiler Lines

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